Isoelectric focusing of B-lactamases on cellulose acetate membranes
- 1 August 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 12 (2) , 193-196
- https://doi.org/10.1093/jac/12.2.193
Abstract
Cellulose acetate membranes treated with boron trifluoride in methanol were found to provide suitable support material for isoelectric focusing of β-lactamases; the method used is described. A linear pH gradient was obtained and the application of visible pi markers allowed the pH gradient to be monitored as focusing proceeded. β-Lactamases extracted from antibiotic-resistant coliform organisms focused as sharp, straight bands upon visualization with nitrocefin. Results were known within two hours of applying the β-lactamase extracts. The method allows a very economical use of expensive ampholyte and offers a quick and more simple alternative procedure for the identification of β-lactamases than the conventional polyacrylamide gel method.Keywords
This publication has 1 reference indexed in Scilit:
- Principal beta-lactamases responsible for resistance to beta-lactam antibiotics in urinary tract infectionsAntimicrobial Agents and Chemotherapy, 1980