Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.
- 1 May 2000
- journal article
- Vol. 38 (5) , 2007-9
Abstract
Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.This publication has 8 references indexed in Scilit:
- On the Origins of Congenic MATα and MATa Strains of the Pathogenic Yeast Cryptococcus neoformansFungal Genetics and Biology, 1999
- Determination of ploidy in Cryptococcus neoformans by flow cytometry.1996
- The alpha-mating type locus of Cryptococcus neoformans contains a peptide pheromone gene.Molecular and Cellular Biology, 1993
- Rapid assessment of S. cerevisiae mating type by PCRTrends in Genetics, 1990