In vitro survival ofEchinococcus granulosus protoscolices in several media, at+4 C and +37 C

Abstract
As a first step towards hydatid disease control, the in vitro survival of protoscolices ofEchinococcus granulosus was investigated for various media and temperatures. Higher percentage survival was obtained than previously reported: at 4°C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin. Maximal survival was 30% at 55 days in these conditions. Flame cell activity was the criterion of choice for viability. At 37°C survival rates were lower and morphological changes in protoscolices were observed.