G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs
- 21 October 2005
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 2 (11) , 829-831
- https://doi.org/10.1038/nmeth797
Abstract
Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.Keywords
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