ACTIVE OXYGEN AND ANTI-INFLAMMATORY DRUG - MEASUREMENT BY LUMINOL DEPENDENT CHEMI-LUMINESCENCE

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Abstract
The active oxygen produced from stimulated phagocytic cells emits luminol-dependent chemiluminescence (CL) on reaction with luminol. The active O2 was measured using the CL and the results were compared with those by the LDH[lactate dehydrogenase]-NADH method. Effects of nonsteroidal antiinflammatory drugs (NSAID) on the generation of active O2 were studied by both methods. Rat peritoneal and pleural exudated cells (PEEC and PLEC) emitted strong CL on incubation with zymosan, but that from rat whole blood cells was very weak. Effects of superoxide dismutase, catalase, NaN3 and L-ascorbic acid on the generation of active O2 from rat phagocytic cells were different between CL and LDH-NADH methods. These discrepancies seem to be due to the different kinds of active O2 that can be measured by both methods. Except for BW-755C, most of the NSAID had only a slight inhibitory effect on the generation of active O2 measured by both methods, and the ex-vivo effect was the same as that observed in vitro. NSAID apparently decrease the phagocytic function of cells by nonspecifically stabilizing the biological membrane and inhibit slightly the generation of active O2 from phagocytes. The CL method could be performed not only in PEEC and PLEC, but also in whole blood cells. CL measurement may be used as a simple and valuable method for the detection of all types of active O2, including superoxide anion radical and its metabolites, and for testing cellular functions and drug actions on them.