Glycopeptides were prepared by pronase digestion from membranous proteins of microsomes of an ascites hepatoma, AH 66, then fractionated to produce neutral and acidic glycopeptide fractions. The neutral fraction behaved nearly homogeneously on electrophoresis and contained only mannose and glucosamine as carbohydrate constituents, its composition being very similar to that of corresponding glycopeptides from rat liver microsomes. The acidic fraction was very heterogeneous, producing several peaks on column chromatography using DEAE-Sepahdex. Every peak contained neutral sugars (galactose, mannose, and fucose), glucosamine, and sialic acid, and the molar ratios of these constituents differed from one peak to another. Each peak was different from the corresponding glycopeptides from rat liver microsomes in its carbohydrate composition.