Comparison of Two Superfusion Systems for Study of Neurotransmitter Release from Rat Cerebral Cortex Slices
- 1 July 1984
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 43 (1) , 236-242
- https://doi.org/10.1111/j.1471-4159.1984.tb06701.x
Abstract
Depolarization-induced release of [3H]GABA and [3H]noradrenaline [norepinephrine] (NA) from rat cerebral cortex slices was studied in 2 superfusion systems: 1 with stationary and the other 1 with continuously shaken slice compartments. Ca-dependent depolarization-induced release of GABA and NA could be demonstrated only with shaken slices. GABA, but not NA, could also be released by high K+ media and veratridine from stationary slices. Synaptic transmitter releasing mechanisms are apparently damaged in stationary slices, possibly due to impaired energy metabolism.Keywords
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