Rooting of tissue cultured camellias

Publisher Website
Google Scholar
Abstract
Summary In order to obtain optimum conditions for in vitro propagation of Camellia japonica in its juvenile growth phase, several rooting experiments were performed. Dipping the basal ends of shoots in concentrated IBA solutions (0.5 and 1 g l−1) for short periods gave much better results than including the auxin in the rooting medium. Roots induced by IBA were better formed than when NAA was used. In general, the 1 g 1−1 IBA level gave better results than 0.5 g l−1 as regards both rooting rates and the number of roots per rooted shoot. Dipping times of 20–30 min were suitable although pronounced clonal differences were observed. In experiments on the sucrose concentration in the rooting medium, the best results were obtained using 30–50 g l−1, depending on the clone. Both rooting rates and the speed of root formation increased when cultures were incubated in the dark for 9–18 days prior to illuminated incubation. This fact must be related to the root initiation phase since the differentiation of root primordia, in in vitro conditions, takes place at about day 16. The reduction by half of Murashige and Skoog macro-nutrients caused the production of more roots than in the undiluted MS medium.

This publication has 1 reference indexed in Scilit: