Antioxidant Functions Required for Insusceptibility of Saccharomyces cerevisiae to Tetracycline Antibiotics
Open Access
- 1 October 2001
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 45 (10) , 2939-2942
- https://doi.org/10.1128/aac.45.10.2939-2942.2001
Abstract
Cu,Zn superoxide dismutase (Sod1) is required for insusceptibility of Saccharomyces cerevisiae to oxytetracycline (OTC). Here we report that Sod1 is also required for insusceptibility to doxycycline (DOX). Furthermore, among a range of antioxidant and redox balance mutants, mac1 and ctr1 deletion strains exhibited marked sensitization to OTC and DOX. Certain mutants exhibited a slight sensitivity to methacycline and minocycline. Addition of copper suppressed antibiotic sensitivity. Thus, intracellular copper as well as superoxide dismutase can be critical for eukaryotic tolerance of several tetracycline antibiotics.Keywords
This publication has 28 references indexed in Scilit:
- Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial ResistanceMicrobiology and Molecular Biology Reviews, 2001
- Metal toxicity in yeasts and the role of oxidative stressAdvances in applied microbiology, 2001
- Yeast Lacking Cu-Zn Superoxide Dismutase Show Altered Iron HomeostasisPublished by Elsevier ,2000
- Undetectable Intracellular Free Copper: The Requirement of a Copper Chaperone for Superoxide DismutaseScience, 1999
- Tetracyclines: antibiotic action, uptake, and resistance mechanismsArchiv für Mikrobiologie, 1996
- Characterization of three yeast copper-zinc superoxide dismutase mutants analogous to those coded for in familial amyotrophic lateral sclerosis.Proceedings of the National Academy of Sciences, 1994
- Molecular characterization of a copper transport protein in S. cerevisiae: An unexpected role for copper in iron transportCell, 1994
- [10] Analysis and manipulation of yeast mitochondrial genesPublished by Elsevier ,1991
- Hydrixyl radical generation by the tetracycline antibiotics with free radical damage to DNA, lipids and carbohydrate in the presence of iron and copper saltsFree Radical Biology & Medicine, 1988
- Structure–Activity Relationships in the Tetracycline SeriesAdvances in applied microbiology, 1970