SPONTANEOUS CYTO-TOXICITY OF HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS FOR THE LYMPHOBLASTOID CELL-LINE CCRF-CEM - AUGMENTATION BY BACTERIAL LIPOPOLYSACCHARIDE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 46  (3) , 640-648
Abstract
Spontaneous cell-mediated cytotoxicity (SCMC) of human peripheral blood mononuclear cells for a poor target, CCRF-CEM, a human lymphoblastoid cell line, was rapidly and markedly elevated by Escherichia coli lipopolysaccharide (LPS). SCMC for K562, a human leukemic myeloid cell line sensitive to SCMC, was only slightly elevated by LPS. The cytotoxicities of both adherent and non-adherent mononuclear cells for CCRF-CEM were elevated. A response to LPS was found in Fc.gamma. receptor-positive and Fc.gamma. receptor-negative T cells. LPS increased the binding of non-adherent cells to both targets, but analysis of the binding suggests that a subsequent step, either triggering of the cytotoxic mechanisms or susceptibility of the target, was the basis for the increased SCMC.

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