A Sensitive and Reproducible Assay for the Quantitation of the MIF Correlate of Delayed Hypersensitivity in Man

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Abstract
An improved migration inhibitory factor (MIF) assay procedure is described which was developed for the study of tumor patient immunocompetence testing both on the clinical level and as an adjunct to basic studies of tumor immunology and the transfer of immunological capacity through immuno-genic RNA. A detailed description of a new migration chamber in which as many as eight capillaries can be simultaneously subjected to identical environmental conditions is presented, This feature reduces the variation among replicate tests to the point where significance can be ascribed to migration differences of ten percent or less. This chamber, along with the use of a cultured human lymphoblastoid tumor cell as the indicator cell, results in a very flexible, convenient, and sensitive direct or indirect assay for the detection of human migration inhibitory factor.

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