DETECTION OF HBS ANTIGEN IN ROUTINE PARAFFIN EMBEDDED LIVER-TISSUE BY ENZYME-LABELED ANTIBODY TECHNIQUE

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 30  (1) , 69-73
Abstract
Hepatitis B surface antigen (HBs Ag) was detected using the enzyme-labelled antibody technique on routinely processed liver biopsy material. Of 85 examined specimens, 45 cases were HBs Ag positive by the immunofluorescent test and the enzyme labelled antibody technique. The remaining 40 cases were negative by both techniques. The specificity of HBs Ag detected by the enzyme-labelled antibody technique was confirmed by the blocking test using guinea pig specfic HBs antibody. The enzyme-labelled antibody technique may be useful for detecting HBs Ag on routine paraffin sections.

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