Structure-Activity Analysis of Microcin J25: Distinct Parts of the Threaded Lasso Molecule Are Responsible for Interaction with Bacterial RNA Polymerase
Open Access
- 1 June 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (11) , 3859-3863
- https://doi.org/10.1128/jb.187.11.3859-3863.2005
Abstract
Peptide microcin J25 (MccJ25) inhibits bacterial RNA polymerase. We show that thermolysin-cleaved MccJ25 and MccJ25 lacking amino acids 13 to 17 also inhibit transcription. Our data and structural analysis of intact and thermolysin-digested MccJ25 suggest that distinct regions of MccJ25 are involved in transcription inhibition and cell entry.Keywords
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