Allergenic components of isolates ofcladosporium herbarum

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Abstract
Six isolates of Cladosporium herbarum, IMI 49630, IMI 46932, IMI 96220, CBS 121.47, CBS 180.50 and ATCC 38810 were grown on synthetic revised tobacco medium for 28 days. Extracts of the mycelia were prepared and their biochemical and immunological properties were examined. Two isolates, IMI 49632 and CBS 180.50 did not give a significant yield of mycelial mat, and the mycelial extract of ATCC 38810 was only weakly immunoreactive. In IgE immunoblots of sodium dodecylsulphate polyacrylamide electrophoresis gels, the remaining three extracts showed strongly reactive bands at 14, 17, 25, 35, 41,47 and 97 kDa MW and IMI 96220 had two additional bands at 53 and 71 kDa. These extracts had 8 to 10 bands in isoelectric focusing with pl values between 3.75 to 5.2, the two predominant bands being at 4.5 and 5.2. In crossed — radioimmunoelectrophoresis using human atopic sera, IMI 96220 showed one dominant and three minor allergens; IMI 49630 and CBS 121.47 had one dominant and one minor allergen. In direct radioallergosorbent tests with human atopic sera and passive cutaneous anaphylaxis tests with mouse IgE, IMI 49630 and 96220 were more potent than CBS 121.47. These results indicate that mycelial extracts of IMI 49630 and 96220 contain a potent but complex mixture of the major allergens of C. herbarum and are preferred sources for a reference standard for this organism.