Sex determination of bovine embryos using the polymerase chain reaction
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Animal Biotechnology
- Vol. 1 (2) , 121-133
- https://doi.org/10.1080/10495399009525735
Abstract
One or two cell biopsies were obtained from 6‐7 days old bovine embryos. The sex of the embryos was determined with two different bovine Y‐chromosome‐specific primer pairs by using the polymerase chain reaction. These results were confirmed by karyotyping as well as in situ hybridization with an independent bovine Y‐chromosome‐specific sequence. The polymerase chain reaction was found to be a quick and accurate method of sex diagnosis of bovine preimplantation embryos.Keywords
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