Isolation of Thioguanine Resistant Variants of K-BALB Cells Non-inducible for Type C Viruses by 5-Iododeoxyuridine
- 1 March 1978
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 38 (3) , 419-429
- https://doi.org/10.1099/0022-1317-38-3-419
Abstract
Clones of thioguanine resistant K-BALB mouse [fibroblast] cells were isolated which were inducible for endogenous type C virus synthesis by cycloheximide and dexamethasone, but not 5-iododeoxyuridine. A comparison of the number of foci formed on [normal rat kidney] NRK and [mouse embryo] SC-1 cells suggested that the xenotropic virus was suppressed. The variants were not defective in the incorporation of thymidine or iododeoxyuridine or deficient in thymidine kinase, but were deficient in hypoxanthine-guanine phosphoribosyltransferase and the incorporation of hypoxanthine into nucleic acid. Because these cells are blocked at some point in the expression of endogenous virus, they may prove useful in establishing the steps involved in chemical activation of virus synthesis.This publication has 2 references indexed in Scilit:
- Suppression of the Avian Sarcoma Virus Genome in 8-Azaguanine-Resistant, Transformed, Hamster Cells1JNCI Journal of the National Cancer Institute, 1976
- Effect of 5-bromodeoxyuridine on nucleic acid and protein synthesis and viability in HeLa cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967