Monitoring the amplification of CATCH, a 3SR based cooperatively coupled isothermal amplification system, by fluorimetric methods
- 15 November 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (22) , 4697-4699
- https://doi.org/10.1093/nar/25.22.4697
Abstract
Three different types of fluorescence detection methods were employed to monitor amplification of a previously established isothermal cooperatively coupled amplification system as it can serve as a tool for the investigation of fundamental issues in evolutionary optimization. By using 5'IRD-41 fluorescent labeled primers, the intercalating dye TOPRO-1 and a 5'fluorescin/3'DABCYL 4-(4-dimethylamino-phenylazo)benzoic acid labeled ss 24 nt DNA, evolving molecular cooperation is accessible, sequence specifically as well as non-sequence-specifically without using radioactivity.Keywords
This publication has 6 references indexed in Scilit:
- A closed tube format for amplification and detection of DNA based on energy transferNucleic Acids Research, 1997
- Cooperative Amplification of Templates by Cross‐Hybridization (CATCH)European Journal of Biochemistry, 1997
- Molecular Beacons: Probes that Fluoresce upon HybridizationNature Biotechnology, 1996
- [13] Purification and characterization of human immunodeficiency virus type 1 reverse transcriptasePublished by Elsevier ,1995
- Strand displacement amplification as an in vitro model for rolling-circle replication: deletion formation and evolution during serial transfer.Proceedings of the National Academy of Sciences, 1994
- Nucleic acid sequence-based amplificationNature, 1991