Fixation and Electron Microscopy of the Rothamsted Culture of Henbane Mosaic Virus
- 1 November 1971
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 13 (2) , 357-359
- https://doi.org/10.1099/0022-1317-13-2-357
Abstract
Thin sections of tissue infected by viruses of the potato virus Y (PVY) group often fail to show virus particles. Henbane mosaic virus (HMV) is more concentrated in infected tissue than most PVY group viruses and seemed a suitable subject for electron microscopy. We failed to detect any particles after tissues were subjected to a commonly used double fixation technique but found them in tissue fixed with osmic acid; parallel results were found when purified virus preparations were similarly treated.Keywords
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