Restoration of Ca2+-Activated Tension of CDTA-Treated Single Skeletal Muscle Fibers by Troponin C1
- 1 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 104 (6) , 873-874
- https://doi.org/10.1093/oxfordjournals.jbchem.a122573
Abstract
Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+ tension development. The addition of troponin C restored the Ca2+ -activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+ -activated ATPase of CDTA-treated rabbit skeletal myofibrils (Morimoto, S.& Ohtsuki, I.(1987) J. Biochem. 101, 291-301).This publication has 6 references indexed in Scilit:
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