Biochemical characterization of Ia antigens encoded by theRT1.B andRT1.D loci in the rat MHC

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Abstract
Ia antigens in rats are genetically associated with typical MHC-linked immune response (Ir) genes. One ratIr gene (IR-GLT) has recently been mapped to theRT1.D locus in a rat MHC recombinant, WRC. We have studied the expression of la antigenic determinants in WRC and its parental strains BN and WRA using a panel of monoclonal antibodies. Our results suggest that the inheritance of1R-GLTcorresponds with the inheritance of I-E-like, but not I-A-like, antigens in the WRC rat. These observations were confirmed when WRC I-E-like antigens were analyzed by two-dimensional gel electrophoresis. We propose thatRT1.D shares both functional and antigenic homologies with the mouseI-E subregion.