Cytological sub-division of S-phase in the Syrian hamster (Mesocricetus auratus)

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Abstract
Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes. — Applied to asynchronous populations successively sampled through one cell cycle, cells in S can be “unscrambled” and replaced in their correct time sequence. — This helps to overcome the sampling-time variable inherent in such populations, and allows a clearer picture of the progression of events both qualitatively and quantitatively.