Transmission of Integrated Sea Urchin Histone Genes by Nuclear Transplantation in Xenopus laevis
- 1 July 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 221 (4605) , 67-69
- https://doi.org/10.1126/science.6857265
Abstract
Sea urchin histone genes contained in a recombinant plasmid pSp102 were microinjected into the cytoplasm of fertilized eggs of Xenopus laevis . By the late blastula stage, plasmid DNA sequences were detected comigrating with the high molecular weight cellular DNA (greater than 48 kilobases). Analysis of the DNA from injected embryos digested with various restriction endonuclease demonstrated that the injected DNA was integrated into the frog genome. Clones of embryos containing the pSp102 DNA sequences were produced by means of nuclear transplantation. Individuals of the same clone contain the pSp102 sequences integrated into similar chromosomal locations. These sites vary between different clones.This publication has 16 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Transposition of Cloned P Elements into Drosophila Germ Line ChromosomesScience, 1982
- Analysis of the Mechanisms Involved in Gene Regulation and Cell Differentiation by Microinjection of Purified Genes and Somatic Cell Nuclei into Amphibian Oocytes and EggsDifferentiation, 1982
- Integration and Stable Germ Line Transmission of Genes Injected into Mouse PronucleiScience, 1981
- GENE TRANSFER IN AMPHIBIAN EGGS AND OOCYTESAnnual Review of Genetics, 1981
- Introduction of a rabbit β-globin gene into the mouse germ lineNature, 1981
- Microinjection of cloned retroviral genomes into mouse zygotes: integration and expression in the animalNature, 1981
- Persistence and expression of histone genes injected into Xenopus eggs in early developmentNature, 1981
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- The organization of sea urchin histone genesCell, 1975