Long-Range1H-15N Heteronuclear Shift Correlation at Natural Abundance: a Tool To Study Benzothiazole Biodegradation by TwoRhodococcusStrains
- 1 April 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (4) , 1412-7
- https://doi.org/10.1128/aem.67.4.1412-1417.2001
Abstract
The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains ofRhodococcuswas monitored by reversed phase high-pressure liquid chromatography and by1H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range1H-15N heteronuclear shift correlation without any previous15N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole.Keywords
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