SOME ELECTROPHORETIC AND IMMUNOLOGICAL PROPERTIES OF HUMAN SEMEN

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Abstract
Cellulose acetate or disk electrophoresis of human semen and seminal plasma revealed between 7 and 10 zones of migration, depending on the technique used. Disk electrophoresis of washed human spermatozoa resulted in a pattern of protein separation that could not be distinguished from those obtained by disk electrophoresis of semen or seminal plasma. This is evidence that proteins being removed from spermatozoa are very similar to those present in seminal plasma. Immunological properties of human semen were investigated by immunological double diffusion and immuno-electrophoresis. The latter technique demonstrated at least 6 antigenic components in semen. These could be resolved into 4 antigens common with serum and 4 antigens specific to semen. Immuno-electrophoresis resolved at least 10 semen components. These were subdivided into 5 common to serum and 5 specific to semen. Finally, there is limited evidence to indicate a substance common between semen and saliva but absent in serum.

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