The predominant form of mammalian DNA topoisomerase Iin vivo has a molecular mass of 100 kDa
- 1 March 1994
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 19 (2) , 99-103
- https://doi.org/10.1007/bf00997154
Abstract
DNA topoisomerase I was purified to apparent homogeneity from human HeLa cells as a single polypeptide with a molecular mass of 100 kDa, as assayed by both gel filtration column chromatography and SDS-polyacrylamide gel electrophoresis. No smaller forms of the enzyme were detected in the purified fraction. Therefore, smaller forms, which have been observed by other investigators, are likely to be the result of proteolysis during isolation and are not relevant to thein vivo activity of DNA topoisomerase I.Keywords
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