Rapid method for identification of gram-negative, nonfermentative bacilli

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Abstract
A rapid system (OA) based on oxidative attack of substrates was developed for [diagnostic] identification of gram-negative, nonfermentative bacilli (NFB). Strains of NFB (112) from 25 spp. (representing the genera Pseudomonas, Alcaligenes, Acinetobacter, Bordetella, Flavobacterium, Moraxella, and Xanthomonas) were assayed by OA, buffered single substrate, and oxidative/fermentative methods. The 38 substrates consisted of salts of organic acids, N-containing compounds, alcohols and carbohydrates. Of the test strains, 94% were identified by the OA method in 24 h, and 99% were identifiable in 48 h. Reproducibility was 99%. Correlation with buffered single substrate was 98% (all substrates) and 90% with the oxidative/fermentative method (carbohydrates only). Biochemical profiles of all strains and tables showing the most useful tests for identification are presented.