A METHOD OF ISOLATION OF PROTEINS FROM DILUTE SOLUTIONS

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Abstract
A method has been developed for the concentration of dilute solutions of biocolloids. It consists of the removal of water by pervaporation and the simultaneous removal of salts and other low molecular weight materials by dialysis. A dilute solution of serum (2 ml. of serum per liter of 0.9% sodium chloride solution) of known composition was used to test the efficacy of the method. The electrophoretic patterns of the reconstituted serum protein solutions were compared with those of the untreated sera. The total recovery of biocolloids from such solutions was about 73% and the electrophoretic patterns obtained were similar to those of the untreated sera. The method is reasonably simple, rapid, and compares favorably with other methods used for the concentration of biocolloids. It has also been employed in the concentration of biocolloids of normal human urine.