Über die Thymo-polynucleotidase aus Pankreas. Nucleinsäuren. V

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Abstract
Thymopoly-nucleotidase, extracted from cattle and hog pancreas with diluted H2SO4 and purified by (NH4)2SO4 fractionation, could be concentrated 60 times. It was most stable at pH 6 and was destroyed by mineral acids, heat, and certain salting out procedures. It hydrolyzed [alpha]-nucleic acids to oligonucleotides (less than 4 nu-cleotide units), liberating one acid group for each tetranucleotide molecule; the enzyme could be detd. by this increase in acidity. Yeast nucleic acid had larger molecules than would correspond to a tetranucleotide.