Effets cytologiques de la Mitomycine C sur des fibroblastes normaux cultivés in vitro

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Abstract
The cytological effects of mitomycin C were studied in vitro on chick embryo fibroblasts and on myoblasts. In the chosen concentrations, growth and mitotic activity are inhibited. Nevertheless, the mitotic index is still elevated, and there exists a significant increase of metaphase forms. This observation which has been confirmed by other investigators with the technique of phase contrast microscopy of living cells, points towards the existence of a blocking effect in metaphase with scattering of chromosomes or parts of chromosomes through the cytoplasm. The cells can either degenerate or survive their mitosis in an abnormal fashion (aberrant chromosomes, absence of cell diaeresis, formation of double nucleated cells, or of a giant nucleus, or of micro nuclei). The cells of the interphase often have a nucleus with a large middle part, which then opens itself into the cytoplasm or buds out producing small accessory nuclei. In cultures treated with Mitomycin C and subjected to the Feulgen reaction there are nuclei which have a diminished content of DNA; a particular type of nuclear degeneration appears to take place.

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