SPECIFIC IMMUNOREACTIVITY OF HYBRIDOMA-SECRETED MONOCLONAL ANTI-MELANOMA ANTIBODIES TO CULTURED-CELLS AND FRESHLY DERIVED HUMAN-CELLS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (10) , 3602-3609
Abstract
The specificities of monoclonal antibodies against melanoma cells were analyzed using radioimmunoassay, mixed-hemadsorption assay and quantitative absorption on a variety of malignant and nonmalignant cells. Of 6 hybridoma-secreted antibodies, 3 bound to the majority of melanoma cell lines, melanoma tumors and astrocytoma cell lines as well as to all normal and Epstein-Barr virus-transformed lymphocytes tested. The binding pattern coincides with the presence or absence of the DR antigen on human cells. Conversely, 2 other antibodies, 19-19 and Nu4B, detected 2 different antigens common to melanoma and astrocytoma cells only. Cloning of melanoma cells resulted in establishment of DR-positive and DR-negative clones, with the binding of Nu4B antibody retained in all.