Effect of oxygen pressure on glycogen synthesis by rat-liver slices

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Abstract
Glycogen synthesized by rat-liver slices 0. 5mm. thick incubated at 1 atm. O2 pressure in Hastings medium with glucose was localized in the cells of the periphery of the slice. Cells of the interior of this slice do not synthesize glycogen. Inner cells of thin slices (about 0. 3mm thick) can synthesize glycogen when such slices are incubated under the same conditions, but O2 pressures higher than 1 atm. are required if inner cells of slices 0.5 mm or more thick are to be able to synthesize glycogen. Localization of newly synthesized glycogen in rat-liver slices incubated in Hastings medium with glucose does not depend on glucose concentration. Calculation of the minimum O2 pressure required to synthesize glycogen gives values between 0.09 and 0.17 atm. The advantages of high O2 pressures for the study of the synthesis of glycogen and other compounds that require adenosine triphosphate are discussed.