Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor.
- 1 August 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (8) , 1436-1439
- https://doi.org/10.1093/clinchem/25.8.1436
Abstract
We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydrogenase solution method. The coefficient of correlation was r = 0.996. A glucose dehydrogenase reactor made starting with 1 mg (250 U) of enzyme was stable during eight weeks of continuous use, that is, for nearly 3500 tests. This reduced the cost of the assay by at least 50-fold, compared with that for a commercial glucose dehydrogenase test pack method.This publication has 1 reference indexed in Scilit:
- The Preparation and Characterization of Lyophilized Polyacrylamide Enzyme Gels for Chemical Analysis.Analytical Chemistry, 1966