Kinetic studies on the formation of acridine‐linked DNA triple helices
- 9 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 357 (3) , 312-316
- https://doi.org/10.1016/0014-5793(94)01387-g
Abstract
We have used DNase I footprinting to measure the rate of intermolecular triple helix formation at the target sites A6G6 · C6T6 and G6A6 · T6C6 with the acridine-linked oligonucleotides Acr-T5C5, and Acr-C5T5 respectively. Under pseudo first-order reaction conditions we find that the reactions are slow, with half-lives of several minutes. The rates are dependent on the concentration of the third strand oligonucleotide and yield bimolecular association rate constants of 300 M−1 · s−1 for Acr-T5C5 binding to A6G6 · C6T6 and 2000 M−1 · s−1 for the interaction of Acr-C5T5 with G6A6 · T6C6.Keywords
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