Protease-catalyzed semisynthesis of human neuropeptide Y.
- 1 January 1989
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 37 (3) , 811-812
- https://doi.org/10.1248/cpb.37.811
Abstract
Human neuropeptide Y was semisynthesized by enzymatic condensation of des-Tyr36-NH2 human neuropeptide Y and H-Tyr-NH2 using Pseudomonas aeruginosa elastase, a metalloenzyme possessing a hydrolytic specificity for the imino side of hydrophobic amino acids. The optimum pH for this enzymatic synthesis was judged to be around 7 in a high concentration of an organic solvent.This publication has 4 references indexed in Scilit:
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- Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine.Proceedings of the National Academy of Sciences, 1984
- Peptide Bond Synthesis Catalyzed by ThermolysinThe Journal of Biochemistry, 1980
- Specific nature of hydrolysis of insulin and tobacco mosaic virus protein by thermolysinArchives of Biochemistry and Biophysics, 1966