A mouse locus at which transcription from both DNA strands produces mRNAs complementary at their 3′ ends
- 1 July 1986
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 322 (6076) , 275-279
- https://doi.org/10.1038/322275a0
Abstract
The organization and large size of the mammalian cell genome allows spatial separation of different transcription units. In those cases where more than one species of messenger are synthesized from the same cellular DNA sequence, they have been found to be generated from transcription proceeding in the same direction. These mRNAs always share regions of homology and can differ from one another as a result of differential processing (splicing and/or polyadenylation) or alternative initiation1–14. In contrast, complementary mRNAs transcribed from opposite strands of the same cellular DNA sequence have not previously been observed. Here we have identified a region of mouse DNA at which processed mRNAs from two adjacent convergent transcription units overlap by 133 base pairs (bp) at their 3′-untranslated ends. One of the transcription units appears to encode a second mRNA which does not contain this overlapping region. This represents the first description of the natural occurrence of processed mammalian cell mRNAs transcribed from opposite strands of the same DNA sequence. The implications of these complementary regions in normal gene regulation are discussed in the context of the finding that the artificial introduction into cells of DNA constructs synthesizing anti-sense RNAs complementary to regions of mRNA transcribed from a chromosomal gene, can inhibit the gene's activity, presumably by the formation of double-stranded RNA15–19.Keywords
This publication has 48 references indexed in Scilit:
- Alternative splicing accounts for the four forms of myelin basic proteinCell, 1985
- Structure of the Human Interleukin-2 Receptor GeneScience, 1985
- Human aldolase isozyme gene: the structure of multispecies aldolase B mRNAsNucleic Acids Research, 1985
- A novel mechanism of alternative RNA splicing for the developmentally regulated generation of troponin T isoforms from a single geneCell, 1984
- Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processingNature, 1983
- Multiple mRNA species with distinct 3′ termini are transcribed from the β2-microglobulin geneNature, 1983
- Multiple polyadenylation sites in a mouse α-amylase geneNucleic Acids Research, 1981
- Size heterogeneity in the 3′ end of dihydrofolate reductase messenger RNAs in mouse cellsCell, 1980
- Two mRNAs can be produced from a single immunoglobulin μ gene by alternative RNA processing pathwaysCell, 1980
- Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3′ endsCell, 1980