Replication of DNA containing 5-bromouracil can be mutagenic in Syrian hamster cells.
Open Access
- 1 November 1984
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (11) , 2449-2454
- https://doi.org/10.1128/mcb.4.11.2449
Abstract
A new protocol for inducing mutations in mammalian cells in culture by exposure to the thymidine analog 5-bromodeoxyuridine (BrdUrd) was established. This protocol, called "DNA-dependent" mutagenesis, involved the incorporation of BrdUrd into DNA under nonmutagenic conditions and the subsequent replication of the 5-bromouracil (BrUra)-containing DNA under mutagenic conditions but with no BrdUrd present in the culture medium. The mutagenic conditions were induced by allowing BrUra-containing DNA to replicate in the presence of high concentrations of thymidine. This generated high intracellular levels of dTTP and dGTP, causing nucleotide pool imbalance. The mutagenesis induced by this protocol was found to correlate with the level of BrUra substituted for thymine in DNA.This publication has 23 references indexed in Scilit:
- DNA base sequence changes induced by bromouracil mutagenesis of lambda phageJournal of Molecular Biology, 1982
- Bromodeoxyuridine mutagenesis in mammalian cells is stimulated by thymidine and suppressed by deoxycytidineNature, 1978
- Bromodeoxyuridine mutagenesis in mammalian cells: mutagenesis is independent of the amount of bromouracil in DNA.Proceedings of the National Academy of Sciences, 1978
- Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1976
- Mutagenic effect of BUdR in diploid human fibroblastsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- In vitro cultures of a pigmented hamster melanoma cell lineExperimental Cell Research, 1964
- Identification of the altered bases in mutated single-stranded DNAJournal of Molecular Biology, 1964
- REVERSAL OF MUTANT PHENOTYPES BY 5-FLUOROURACID: AN APPROACH TO NUCLEOTIDE SEQUENCES IN MESSENGER-RNAProceedings of the National Academy of Sciences, 1962
- Mutation as an error in base pairing. I. The mutagenicity of base analogues and their incorporation into the DNA of Salmonella typhimurium.1961
- THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVESProceedings of the National Academy of Sciences, 1958