Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains
- 1 September 1989
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 107 (3-4) , 315-322
- https://doi.org/10.1007/bf01317927
Abstract
Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.Keywords
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