GENETIC STUDIES ON AMYLASE OF DIFFERENT ELECTROPHORETIC MOBILITY PRODUCED BY STRAINS OF BACILLUS SUBTILIS
- 1 January 1967
- journal article
- research article
- Published by Genetics Society of Japan in The Japanese Journal of Genetics
- Vol. 42 (4) , 251-261
- https://doi.org/10.1266/jjg.42.251
Abstract
Twelve amylase negative mutants were isolated from B. subtilis 168 and its derivatives by irradiating spores with ultraviolet light. Using these mutants as recipients, genetic analysis of the gene controlling the production of amylase in B. subtilis was performed by DNA transformation. An agar gel electrophoretic study showed that genealogically different strains carried different amylase genes resulting in the production of amylases with different electrophoretic mobility. These amylase genes are probably allelic to each other and a site named E within the gene structure specifies the molecular forms of each amylase protein.Keywords
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