A Note on Determining Amylopectin A‐to‐B Chain Ratios

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Abstract
The A‐to‐B chain ratio is one method being used to characterize amylopectin. We found it difficult to obtain reproducable A‐to‐B chain ratios. The major problem appears to be related to the series of mathematical steps involved in the calculations rather than variations in the primary data. Small changes inNelsonreducing power gave relatively large differences in A‐to‐B ratios. Determination of the standard deviation in determining reducing power at various glucose concentrations followed by calculation of theoretical A‐to‐B chain ratios and their corresponding 95% confidence internals showed that reducing power must be determined with extreme precision if the A‐to‐B ratios are to be meaningful.

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