Use of Fluorescent Antibody Techniques in Observations on the Morphogenesis of Fungi

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Abstract
Observations on cells of Candida albicans stained with fluorescein-labelled antibody indicate that wall material of the parent cell is incorporated into the wall of daughter cells or into hyphal walls when these are produced. A diminution in fluorescent intensity along the length of the newly formed hyphae was apparent, suggesting a dilution of parent wall material with continued growth. Fluorescence of newly formed elements was observed when the cells were grown in nonlabelled immune serum, ruling out the possibility that these cells became stained by possibly dissociated antibodies. Conidia of Fusarium oxysporum f. cubense stained intensely, but newly emerged germ tubes failed to do so. Sites of germ tube emergence were readily apparent as nonfluorescing areas of the conidium wall. When young colonies were treated with FITC labelled serum, the phialide tips, the microconidia, and macroconidia all stained intensely with antisera prepared against microconidia, while hyphal walls failed to react with the stain, suggesting an antigenic dissimilarity in the walls of the hyphae and conidia.

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