Microarray-based genomic selection for high-throughput resequencing
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- 14 October 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (11) , 907-909
- https://doi.org/10.1038/nmeth1109
Abstract
We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.Keywords
This publication has 16 references indexed in Scilit:
- Multigene amplification and massively parallel sequencing for cancer mutation discoveryProceedings of the National Academy of Sciences, 2007
- The Consensus Coding Sequences of Human Breast and Colorectal CancersScience, 2006
- Targeted, haplotype-resolved resequencing of long segments of the human genomeGenomics, 2005
- Accurate Multiplex Polony Sequencing of an Evolved Bacterial GenomeScience, 2005
- Genome sequencing in microfabricated high-density picolitre reactorsNature, 2005
- Whole-Genome Patterns of Common DNA Variation in Three Human PopulationsScience, 2005
- Microarray-based resequencing of multiple Bacillus anthracisisolatesGenome Biology, 2004
- Advanced sequencing technologies: methods and goalsNature Reviews Genetics, 2004
- Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using YeastGenome Research, 2002
- High-Throughput Variation Detection and Genotyping Using MicroarraysGenome Research, 2001