Influence of Deoxyribonucleic Acid Degradation Products and Orthophosphate on Deoxynucleotide Kinase Activity and Deoxyribonucleic Acid Synthesis in Pneumococcus Type III

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Abstract
An oligodeoxynucleotide fraction derived from a deoxyribonuclease -treated calf-thymus deoxyribonucleic-acid (DNA) can enhance the activity of deoxycytidylic acid (dCMP) and de-oxyguanylic acid (dGMP) kinases in cell suspensions of type-III pneu-mococci. High levels of orthophosphate can produce similar effects. For part of the incubation period, the activity of dCMP and dGMP kinases is very low or undetectable in unsupplemented-cell suspensions of pneumococci. In contrast, the remaining kinases, deoxyadenylic acid and thymidylic acid, which are present in ample amounts in control and supplemented cells throughout the incubation period, are unaffected by the addition of oligodeoxynucleotides and orthophosphate. The stimulation of kinase activity is amino -acid-dependent and can be abolished by adding chloramphenicoL When the oligodeoxynucleotide fraction and orthophosphate are further supplemented with all eight of the naturally occurring deoxynucleosides and deoxynucleotides (which do not affect kinase activity), a preferential enhancement of DNA synthesis occurs in comparison with cell growth or protein synthesis. Addition of deoxynucleosides and deoxynucleotides to unsupplemented cells produces only a slight increase in DNA synthesis. The preferential enhancement of DNA synthesis can be prevented by adding chloramphenicol at a certain time during incubation.