Detection of new MHC mutations in mice by skin grafting, tumor transplantation and monoclonal antibodies: a comparison.
Open Access
- 1 February 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 118 (2) , 287-298
- https://doi.org/10.1093/genetics/118.2.287
Abstract
Two mechanisms of major histocompatibility complex (MHC) mutations have been described in mice; gene conversion and homologous but unequal recombination. However, our knowledge of mutations in MHC is incomplete because studies have been limited almost exclusively to two haplotypes, H-2b and H-2d, while hundreds of haplotypes exist in nature; it has been biased by the use of only one procedure of screening for mutation, skin grafting. We used three procedures to screen for MHC mutations: (1) conventional techniques of skin grafting, (2) syngeneic tumor transplantation and (3) typing with monoclonal anti-MHC antibodies (mAbs) and complement. The faster technique of tumor transplantation detected mutants similar to those discovered by skin grafting technique. Screening with mAbs allowed us to detect both mutants that are capable of rejecting standard skin grafts and those that are silent in skin grafting tests, and which therefore resulted in a higher apparent mutation frequency. Two mutants of the H-2a haplotype were found that carry concomitant class I and class II antigenic alterations. Both MHC mutants silent in skin grafting tests and mutants carrying concomitant class I and class II alterations have never been studied before and are expected to reveal new mechanisms of generating MHC mutations. 1-Ethyl-1-nitrosourea (ENU) failed to induce de novo MHC mutations in male mice in our skin grafting series.This publication has 52 references indexed in Scilit:
- Tables for determining the statistical significance of mutation frequenciesPublished by Elsevier ,2003
- The H-ZKkm1 mutation: Nucleotide sequence and comparative analysisMolecular Immunology, 1987
- Induction of recessive lethal mutations in theT/t–H-2region of the mouse genome by a point mutagenGenetics Research, 1986
- Gene clusters and the evolution of the major histocompatibility systemPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1986
- ANALYSIS OF THE B10.D2‐H‐2d m l MUTANT WITH MONOCLONAL ANTIBODIESInternational Journal of Immunogenetics, 1983
- Clusters of genes encoding mouse transplantation antigensCell, 1982
- Notable diversity in peptide composition of murine H-2K and H-2D alloantigensBiochemistry, 1974
- Histocompatibility mutations in mice: chemical induction and linkage with the H-2 locusGenetics Research, 1972
- Inherited histocompatibility changes in progeny of irradiated and unirradiated inbred miceGenetics Research, 1965
- A RAPID METHOD OF GRAFTING SKIN ON TAILS OF MICEPlastic and Reconstructive Surgery, 1960