UPTAKE OF 3H-CORTISOL BY RABBIT POLYMORPHONUCLEAR LEUKOCYTES IN VITRO

1 August 1971

journal article
Published by Oxford University Press (OUP) in Acta Endocrinologica

Vol. 67 (4) , 634-652
https://doi.org/10.1530/acta.0.0670634

Abstract

The in vitro uptake of ³H-cortisol by rabbit polymorphonuclear leukocytes (PMN) was studied. The ³H-cortisol concentration in the incubation medium was 1 ng/ml. By adding non-radioactive cortisol to the medium the ³H-cortisol uptake could be depressed, indicating a saturable component in the uptake. At 37°C the uptake was maximal within 10 minutes. The uptake was also depressed by progesterone, 17α-OH-progesterone, corticosterone and to a lesser degree by testosterone and 17β-oestradiol. Chromatography showed that almost all the ³H-cortisol was bound in an unchanged form. The uptake and retention were temperature-sensitive. Contrary to the cortisol-binding site of transcortin, the saturable binding of ³H-cortisol in the PMN's was not destroyed by trypsin. The ³H-cortisol uptake in erythrocytes was also studied; it was very small and showed no saturable component. The saturable ³H-cortisol uptake in the PMN's might be related to a special sensitivity to this hormone.

Keywords

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