Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae
- 1 December 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (12) , 2913-2917
- https://doi.org/10.1073/pnas.68.12.2913
Abstract
A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.Keywords
This publication has 18 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition sitePublished by Elsevier ,2004
- Molecular weights of coliphages and coliphage DNAJournal of Molecular Biology, 1970
- A Restriction enzyme from Hemophilus influenzaeJournal of Molecular Biology, 1970
- The Escherichia coli B restriction endonucleaseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- DNA Restriction Enzyme from E. coliNature, 1968
- Electrophoretic separation of viral nucleic acids on polyacrylamide gelsJournal of Molecular Biology, 1967
- Analysis of C14-labeled proteins by disc electrophoresisBiochemical and Biophysical Research Communications, 1965
- Sedimentation Rate as a Measure of Molecular Weight of DNABiophysical Journal, 1963
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961