Comparison of the Kininogenase Activity of Human Pancreatic Trypsins and Porcine Kallikrein on Met-Lys-Bradykinin and Human Plasma Kininogen

Abstract
Human trypsins 1 and 2 [EC 3.4.21.4] both converted Met-Lys-bradykinin to bradykinin and released bradykinin from kininogen in human plasma as measured by bioassay with the isolated guinea pig ileum. Porcine kallikrein [EC 3.4.21.8] did not act on Met-Lys-bradykinin and released kallidin from human kininogen. Since human trypsin 1 is only partially and trypsin 2 completely inhibited by soybean trypsin inhibitor, the criterion of susceptibility to soybean trypsin inhibitor cannot be used to discriminate between trypsin and kallikrein of different species.

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