A bilayer plate technique to detect broad-spectrum antagonism in microorganisms and its application to wood-inhabiting fungi

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Abstract
A simple method is described for measuring antagonistic activity in microorganisms based on the mycelial growth response of up to 10 isolates of the bioassay fungus in a single agar plate. The technique overcomes disadvantages of existing methods by providing quantitative estimates of interstrain variability in the bioassay fungus as well as the antagonist. Application of the method to a wide range of isolates of Ascocoryne sarcoides (Jacq. ex Gray) Groves and Wilson against three species of decay fungi has permitted the rapid detection of broad-spectrum strains of this fungus which would not have been practical by conventional bioassays.