Conditions for production of initiation protein for germination of Clostridium perfringens spores

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Abstract
The amount of initiation protein (IP) produced by Clostridium perfringens was increased by approximately one-third by modification of Duncan–Strong sporulation medium to include 0.5% glucose, 2% proteose peptone, and 2% sodium phosphate. IP but no spores were produced by the wild type when grown at 46 °C and by a mutant blocked at stage 0 of sporulation. IP appears to be a vegetative cell product, perhaps an overproduced autolysin or pathogenicity factor.

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