Chemiluminescence Assay of N-Acetyl-.BETA.-D-glucosaminidase in Urine Using O-Aminophthalylhydrazido-N-acetyl-.BETA.-D-glucosaminide.

1 January 1991

journal article
Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN

Vol. 39 (5) , 1317-1319
https://doi.org/10.1248/cpb.39.1317

Abstract

The chemiluminescence assay of urinary N-acetyl-beta-D-glucosaminidase using o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide as the substrate was examined. Under optimized conditions, a linear chemiluminescence response to the standard N-acetyl-beta-D-glucosaminidase (NAGase) of different concentrations was observed with a correlation coefficient of 0.999 over the range of 0.3 to 20 I.U./l. The present method was compared with the prevalent method using p-nitrophenyl-N-acetyl-beta-D-glucosaminide in the assay of 20 urine samples from healthy subjects, and the correlation coefficient of 0.891 was obtained.

Keywords

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