LETHAL AND CYTOKINETIC EFFECTS OF ANGUIDINE ON A HUMAN COLON CANCER CELL LINE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (10) , 3304-3309
Abstract
Anguidine is a fungal metabolite with antitumor activity in a murine colon cancer model. Because of disappointing results in clinical trials, the lethal and cytokinetic effects of anguidine on cultured human colon cancer [LoVo] cells was studied. The studies revealed a moderate reduction in survival only after prolonged drug exposure. Continuous incubation with anguidine for longer than 48 h produced a moderate increase in the percentage of S-phase cells and a slight decrease in the proportion of cells in G1/0, by pulse cytophotometry. An immediate reduction in the cumulative labeling index for cells continuously exposed to tritiated thymidine and anguidine and a rapid decrease in the cumulative mitotic index for cells continuously exposed to colcemide and anguidine indicated a block at the G1 into S and G2 into mitosis transitions. Tumoricidal activity of anguidine in a cultured human colon cancer line was poor and required prolonged exposure. The kinetic data reflect an almost frozen state of the cell cycle.